Supplementary MaterialsAdditional document 1: ASC characterization methods and results and volcano plots of DEGs between ASCs expanded in FBS and pHPL. gene list for ASCs expanded in FBS between subsequent passages. Complete list of up- and downregulated genes for FBS-ASCs between subsequent passages (P0 – P1, P1 – P2, P2 – P3, P3 – P4, P4 – P5). This data relates to Fig.?2a. (XLSX 88 kb) 13287_2019_1370_MOESM4_ESM.xlsx (88K) GUID:?1843FDBE-577B-4567-A73A-B8D540229064 Additional file 5: Up- and downregulated gene list for ASCs expanded in pHPL between subsequent passages. Complete list of up- and downregulated genes for pHPL-ASCs between subsequent passages (P0 – P1, P1 – P2, P2 – P3, P3 – P4, P4 – P5). This data relates to Fig.?2b. (XLSX 58 kb) 13287_2019_1370_MOESM5_ESM.xlsx (59K) GUID:?DADC3527-22E8-4002-AFE0-AB8C3BBBF97C Additional file 6: Gene ontology terms for ASCs expanded in FBS between subsequent passages. Complete list of enriched GO terms for FBS-ASCs between subsequent passages (P0 – P1, P1 – P2, P2 – P3, P3 – P4, P4 – P5). This data relates to Table ?Table2.2. (XLSX 516 kb) 13287_2019_1370_MOESM6_ESM.xlsx (517K) GUID:?0B7D3050-F2B1-4381-BF9C-5DB9857B6170 Additional file 7: Gene ontology terms for ASCs expanded in pHPL between subsequent passages. Complete list of enriched GO terms for pHPL-ASCs between subsequent passages (P0 – P1, P1 – P2, P2 – P3, P3 – P4, P4 – P5). This data relates to Table?3. (XLSX 349 kb) 13287_2019_1370_MOESM7_ESM.xlsx (350K) GUID:?6FCAC826-CC49-4A9F-AA85-743544520B9B Additional file 8: Up- and downregulated gene list for ASCs expanded in FBS between P0 and subsequent passages. Complete list of up- and downregulated genes for FBS-ASCs BRD9539 between P0 and subsequent passages (P0 – P1, P0 – P2, P0 – P3, P0 – P4, P0 – P5). This data relates to Fig.?3a. (XLSX 166 kb) 13287_2019_1370_MOESM8_ESM.xlsx (167K) GUID:?44E84BF1-52BC-430B-B975-2D33BE41FCAF Additional file 9: Up- and downregulated gene list for ASCs expanded in pHPL between P0 and subsequent passages. Complete list of up- and downregulated genes for pHPL-ASCs between P0 and subsequent passages (P0 – P1, P0 – P2, P0 – P3, P0 – P4, P0 – P5). This data relates to Fig.?3b. (XLSX Pax1 206 kb) 13287_2019_1370_MOESM9_ESM.xlsx (207K) GUID:?B2A48FAA-7666-4934-90E0-B1068349CC47 Additional file 10: Gene ontology terms for ASCs expanded in FBS between P0 and subsequent passages. Complete list of enriched GO terms for FBS-ASCs between P0 and subsequent passages (P0 – P1, P0 – P2, P0 – P3, P0 – P4, P0 – P5). This data relates to Table ?Table4.4. (XLSX 1145 kb) 13287_2019_1370_MOESM10_ESM.xlsx (1.1M) GUID:?02915046-46B2-46E3-865A-98934FF66629 Additional file 11: Gene ontology terms for ASCs expanded in pHPL between P0 and subsequent passages. Complete list of enriched GO terms for pHPL-ASCs between P0 and subsequent passages (P0 – P1, P0 – P2, P0 – P3, P0 – P4, P0 – P5). This data relates to Table ?Table5.5. (XLSX 1289 kb) 13287_2019_1370_MOESM11_ESM.xlsx (1.2M) GUID:?896632C6-EFAD-45EC-B211-C6AC8BF9E850 Additional file 12: FBS and pHPL-ASC passage specific gene expression profile. A complete list of genes comprising the FBS and pHPL-ASC passage specific gene expression profile. (XLSX BRD9539 89 kb) 13287_2019_1370_MOESM12_ESM.xlsx (90K) GUID:?027A624C-BC28-4ED6-A777-68999E7FBE41 Additional file 13: FBS and pHPL-ASC medium supplementation specific gene expression profile. A complete list of genes comprising the FBS and pHPL-ASC medium supplementation specific gene expression profile. (XLSX 67 kb) 13287_2019_1370_MOESM13_ESM.xlsx BRD9539 (68K) GUID:?117BA4D6-3BE8-43A7-B824-F5C748DA321A Additional file 14: ASC gene signature irrespective of cell passage number and/or BRD9539 media supplement used. A complete list of genes comprising the ASC gene signature irrespective of cell passage number and/or press supplement utilized. (XLSX 20 kb) 13287_2019_1370_MOESM14_ESM.xlsx (21K) GUID:?E8C0ABED-2EBE-442C-A774-E220A9CC06DF Data Availability StatementThe datasets helping the conclusions of the content are included within this article (and its own additional documents). The microarray documents of this research will be transferred in NCBI GEO (Gene Manifestation Omnibus). Abstract History For adipose-derived stromal cells (ASCs) to become safe for make use of in the medical setting, they have to prepare yourself using good making methods (GMPs). Fetal bovine serum (FBS), utilized to increase ASCs in vitro in a few human clinical tests, runs the chance of xenoimmunization and zoonotic disease transmitting. To make sure that GMP specifications are taken care of, pooled human being platelet.
Supplementary MaterialsAdditional document 1: ASC characterization methods and results and volcano plots of DEGs between ASCs expanded in FBS and pHPL