The data are representative of three independent experiments
The data are representative of three independent experiments. ALD-DNA and LPS synergize for inducing IgG production in naive B cells After demonstrating that ALD-DNA enhanced LPS-induced survival, proliferation and the up-regulation of CD86 expression, we next analyzed the effect of ALD-DNA around the antibody production by LPS-activated B cells. proliferation, plasma cell generation, and IL-10 production. (A) CFSE-labeled na?ve B cells were stimulated with ssDNA (0 g/mL, 10 g/mL, 50 g/mL, or 100 g/ml) in the presence or absence of 100 ng/ml LPS for 72 hours. The frequency of proliferating (B220+CFSE-low) B cells was determined by performing circulation cytometry analysis. Data, pooled from three impartial experiments, are shown as bar graphs (means SEM, n?=?5). **DNA, and ***DNA. (B) CFSE-labeled na?ve B cells were stimulated with ssDNA (10 g/mL or 50 g/ml) in the presence or absence of 100 ng/ml LPS for 72 h. Cells were analyzed by circulation cytometry for…
The Tip60 PDB file was modified in Maestro 9
The Tip60 PDB file was modified in Maestro 9.0.211 to add hydrogen atoms and remove water molecules and the acetyl-CoA from tip60. pathways, including induction of expression of proapoptotic proteins Bax, Bak, PUMA, Noxa, and Bim, and inhibition of expression of antiapoptotic proteins Bcl-2 and Bcl-XL. Anacardic acid was previously exhibited as an inhibitor of DNA polymerase [28]. To develop inhibitors more specifically targeting the MYST family of HATs, our group recently 2′-Deoxyguanosine reported substrate-based analog compounds for Tip60 inhibition [29]. Although they present good inhibition activities, the negative charges due to the presence of CoA motif imply that this type of inhibitors may have low pharmacokinetic performance [30]. To further develop potent inhibitors of MYST HATs with enhanced pharmacological properties, 2′-Deoxyguanosine in this work, we have conducted a virtual screening based on the crystal structure of Esa1 (the yeast homolog of Tip60) to search for small molecule inhibitors. In…